The EDWARDS LECTURE will take place in Joyce Powell Leadership Room, MU, from 4:00-6:00pm. Below is the abstract provided by Prof. Harry Yeh from Engineering.
Geometrically Enhanced Differential Immunocapture: Using obstacle arrays in microfluidic devices to enhance efficient and pure rare cell capture from fluid suspensions
Presented by Brian J. Kirby, Sibley School of Mechanical and Aerospace Engineering,
In this talk, I will discuss our work on rare cell capture from blood with applications to cancer
diagnosis. My focus will be on microdevice design and the fluid mechanics associated with flowing whole blood through our microdevices. We use an approach that we call Geometrically Enhance Differential Immunocapture (GEDI), which comprises a biologically specific adhesive surface and a microdevice geometry that induces cell-wall collisions that are dependent on physical cell properties such as size, stiffness, or electrical capacitance. By combining physical and biological specificity, we are able to enrich rare cells (for example, circulating tumor cells in cancer patients) from 1 ppb to up to 65%. The geometry of these devices is periodic, and the periodicity allows the design to be informed by Fourier analysis in analogy with undersampling of high-frequency signals. Some aspects of the physics are nonlinear and thus not all of the design can occur in frequency space, but this formalism nonetheless simplifies the design and informs more complicated systems. Our devices are currently in Phase II clinical trials examining pharmacological response to chemotherapy in late-stage prostate cancer, and are also used with breast, gastric, ovarian, and pancreatic cancer patients.